The 162nd Notice of AgriX Forum | Genome of Wheat Variety Kenong 9204 and Molecular Analysis of High Yield and Nitrogen Efficiency
In the last Baibo Wisdom Lecture Hall, researcher Xia Zhiqiang, vice president of the Tropical Crops School of Hainan University, was invited to share the content of "Big Data Breeding Reform in the Post genomic Era". The lecture was very exciting. Welcome to watch the replay.
This is the 162nd issue of Baibo Wisdom Lecture Hall (August 30). Professor Cui Fa of Ludong University was invited to share the content of "Genome and High yield Nitrogen Efficient Molecular Analysis of Wheat Variety Kenong 9204". Welcome to this lecture!
Professor Cui Fa, Ludong University
The 162nd Lecture
Targeted sequencing is a method to isolate, enrich and sequence a group of target genes or genome regions. This method enables researchers to focus time, cost and data analysis on specific regions of interest (target regions, genes), and use less data to obtain higher sensitivity and accuracy, so as to achieve rapid screening of mutation sites. These target regions usually include exome (the protein coding part of the genome), specific genes of interest (customized content), and target regions in genes or mitochondrial DNA.
FBI seq (Foreground and Background Integrated genotyping by sequencing) is not sequencing by the US Federal Bureau of Investigation, but genotyping sequencing integrating foreground and background. As the name implies, this technology realizes the detection and selection of foreground genes and genetic background at the same time. The selection of foreground and background is two very important steps in molecular breeding. At present, breeders often need to carry out these two steps independently: first, screen foreground target sites, and then develop a large number of probes/bait/PCR primers to detect background genotypes. These time-consuming and costly preparations greatly delay the start of breeding projects.
Deeply cultivate the agricultural core, and work together for the future. In response to the call of the country to build a "China core" of seed industry, and to promote the joint construction of the "Northern Seed City", Phoenix Expo was officially upgraded to the "China Northern Seed Expo" on the basis of successfully holding three sessions of China Shandong International Vegetable Seed Expo, focusing on new varieties and new technologies such as field, vegetables, fruits and vegetables, horticulture, balcony agriculture, and combining cloud seed industry, cloud plant protection, cloud agricultural science platform, Build online and offline promotion and trading dual platforms for seed breeding. The 2022 Northern Species Expo will be linked with the 29th Shandong Double Trade Fair for Plant Protection, China Shandong International New Fertilizer Exhibition, and China (Shandong) New Agricultural Equipment Exhibition!